Figure 1.

Phenotypic abnormalities of DNMT3B7 transgenic mice. A, Top, Schematic diagram of DNMT3B, and the DNMT3B7 isoform. Numbers indicate positions of amino acids (aa). N, N-terminus; C, C-terminus; NLS, nuclear localization signal; PWWP domain, conserved proline-tryptophan-tryptophan-proline motif; PHD domain, plant homeodomain. The asterisks represent the five novel amino acids present in DNMT3B7. Bottom, Schematic diagram of the DNMT3B7 transgenic construct. B, Coronal sections of E15.5 wild-type and DNMT3B7 transgenic mice. Red arrowheads indicate the right eye, and green arrowheads indicate the left eye. Black arrowheads indicate the location of the hard palate. C, Hemotoxylin and eosin-stained sections of the ventricles from E14.5 wild-type and DNMT3B7 animals. A black arrowhead indicates the location of a sub-aortic ventricular septal defect in a homozygous transgenic animal, and yellow arrows indicate the presence of a thin myocardium. RV, right ventricle; LV, left ventricle. D, FACS analysis of B lymphocytes and serum immunoglobulin analysis from Line A transgenic animals. The percentage of total B lymphocytes (top) and of mature IgM⁺B220⁺ B lymphocytes (middle) present in the peripheral blood of Line A homozygous versus wild-type animals at P0. Quantitation of IgA levels measured from serum of Line A homozygous versus wild-type animals (bottom). *** denotes P≤0.0003, ** denotes P≤0.009, and * denotes P≤0.01 between wild-type and homozygous animals using the two-tailed Student’s t-test. Values represent mean ± s.e.m.