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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2010 Mar 7;121(1-2):403–407. doi: 10.1016/j.jsbmb.2010.03.009

Figure 1.

Figure 1

(a) Effects of vitamin D compounds on HT29 cell proliferation. Time dependent effects of vitamin D compounds on the mRNA expression for VDR (B), CYP24 (C), and CYP27b1 (D) in HT29 cells. Normalized values for vehicle-treated cells were set to 1 and fold induction by 1,25(OH)2D, 25(OH)D and 1α(OH)D5 are shown. RT-PCR experiments were conducted in triplicates and statistical analysis was determined an ANOVA (***p<0.001, ** p<0.01, ***p<0.05). Western blot analysis in HT-29 cells treated with [1] vehicle; [2] D3 (1 μM); [3] 1,25(OH)2D (0.1 μM); [4] 25(OH)D (1 μM); or [5] 1α(OH)D5 μM). Exposure to vitamin D metabolites resulted in a significant increase proteins levels for (B) VDR and 1α-hydroxylase (D) by 24 hr post treatment. Equal loading for each experiment is represented by separate actin bands.