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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Peptides. 2010 May 12;31(8):1569–1578. doi: 10.1016/j.peptides.2010.04.023

Fig. 4.

Fig. 4

The ability [DTyr6, βAla11,Phe13,Nle14]Bn(6-14) (Peptide # 1) and the putative BRS-3 selective agonist peptide #16a, to stimulate activation of protein kinase D in hBRS-3/N417 cells, hGRPR/HuTu-80 cells and hNMBR/1299 cells. Each of the cells was incubated with the indicated concentration of [DTyr6, βAla11,Phe13,Nle14]Bn(6-14) (Peptide # 1) or peptide #16a for 5 minutes and the activation of PKD was assessed by determining the extent of formation of phospho-PKD (Ser744/748) and the gel loading assessed by determining tubulin. A representative result from three experiments is shown in the top panel. The average result from densitometric analysis of at least 3 experiments (mean ± SEM) is shown in the bottom panel. Results are expressed as the percentage of the control value.