Table 2.
Affinities and potencies of peptides #1 to #4, #16a, GRP and NMB for hBRS-3 expressed in N417 and H1299 cells.
| IC50 (nM) |
EC50 (nM) |
|||
|---|---|---|---|---|
| PEPTIDE NAME/NUMBER |
hBRS-3/N417 |
hBRS-3/H1299 |
hBRS-3/N417 |
hBRS-3/H1299 |
| 16a | 3162±124 | >10,000 | >10,000 | >10,000 |
| 1 | 1.5±0.1 | 0.60±0.03 | 3.3±0.2 | 0.69±0.03 |
| 2 | 4.3±0.2 | 29.5±2.3 | 12.3±0.9 | 10.0±0.9 |
| 3 | 1.4±0.2 | 10.0±0.8 | 33.1±1.9 | 16.6±0.8 |
| 4 | 250±12 | 572±63 | 52.5±3.2 | 398±21 |
| GRP | >10,000 | >10,000 | >10,000 | >10,000 |
| NMB | >10,000 | >10,000 | >10,000 | >10,000 |
Results are calculated from dose-response curves in Fig. 1 as described in Methods. Results are expressed as the peptide concentration causing half-maximal inhibition of binding (IC50) or the peptide concentration causing half maximal stimulation of [3H]IP (EC50). Results are means ± SEM from at least three experiments. Abbreviations: see Table 1.