Table 3.
Affinities and potencies of peptides peptides #1 to #4, #16a, GRP and NMB for hBRS-3, hGRPR and hNMBR expressed in Balb cells.
| IC50 (nM) | EC50 (nM) | |||||
|---|---|---|---|---|---|---|
| PEPTIDE NAME/NUMBER |
hBRS-3/Balb |
hGRPR/Balb |
hNMBR/Balb |
hBRS-3/Balb |
hGRPR/Balb |
hNMBR/Balb |
| 16a | 6026±148 | >10,000 | >10,000 | >10,000 | >10,000 | >10,000 |
| 1 | 0.85±0.05 | 0.07±0.01 | 1.48±0.3 | 1.1 ± 0.1 | 0.45±0.02 | 0.16±0.01 |
| 2 | 3.39±0.15 | 110±5 | 158±8 | 5.9 ± 0.2 | 58±2.0 | 38±1.2 |
| 3 | 2.09±0.06 | 258±6 | 589±32 | 3.6 ± 0.1 | 977±56 | 219±12 |
| 4 | 63.1±3.1 | >10,000 | >10,000 | 21 ± 1 | 1480±89 | 661±27 |
| GRP | >10,000 | 0.17±0.01 | 155±9 | >10,000 | 4.8±0.2 | 933±74 |
| NMB | >10,000 | 45.2±2.8 | 0.79±0.21 | >10,000 | 209±14 | 1.2±0.1 |
Results are calculated from dose-response curves in Fig. 2 as described in Methods. Results are expressed as the peptide concentration causing half-maximal inhibition of binding (IC50) or the peptide concentration causing half maximal stimulation of [3H]IP (EC50). Results are means ± SEM from at least three experiments. Abbreviations: see Table 1.