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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Cancer Res. 2010 Jun 29;70(14):5788–5796. doi: 10.1158/0008-5472.CAN-09-4519

Figure 3. Glycopeptide specific RFT CD4 T cells proliferate to endogenous stimulation in the periphery of MUC1-Tg mice.

Figure 3

3-5×106 CFSE-labeled T cells were transferred (i.v.) to recipient mice (WT, n=6; MUC1-Tg, n=5). 5-7 days following adoptive transfer, recipient mice splenocytes were analyzed by flow cytometry for the presence of RFT CD4 T cells (CD3+CD4+CD45.1+). T cell proliferation is shown as A) percent of RFT CD4 T cells with decreased CFSE fluorescence and B) CFSE mean fluorescent intensity (MFI) of proliferating cells, calculated using the gating strategy in panel A. C) Percent of RFT CD4 T cells with respect to the recipient mouse CD4 T cell population. Mean values are shown (—). Data is representative of two independent experiments. The p values were calculated using an unpaired t test.