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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: Cancer Res. 2010 Jun 29;70(14):5788–5796. doi: 10.1158/0008-5472.CAN-09-4519

Figure 5. Stimulation of foreign antigen-specific CD4 T cells can help break endogenous MUC1p-specific CD4 T cell tolerance.

Figure 5

3-5×106 CFSE-labeled A,B) VFT and RFT T cells (WT, n=4; MUC1-Tg, n=4) or C,D) VFT and OTII T cells (WT, n=3; MUC1-Tg, n=3) were co-transferred (i.v.) to recipient mice (WT and MUC1-Tg). One day later, mice were vaccinated (i.v.) using co-loaded A,B) DC-(MUC1p+TnMUC1) or C,D) DC-(MUC1p+ova). 4-5 days following the vaccination, the spleen of each recipient mouse was analyzed by flow cytometry for the presence of either A) VFT CD4 T cells (Vα2+CD90.1+) and B) RFT CD4 T cells (CD45.1+) or C) VFT CD4 T cells and D) OTII CD4 T cells (CFSE+) as a percentage of total CD3+CD4+ cells. The proliferation of donor cells was determined by a decrease of CFSE fluorescent intensity. Mean values are shown (—).