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. Author manuscript; available in PMC: 2010 Jul 17.
Published in final edited form as: Biochem Biophys Res Commun. 2005 Aug 5;333(3):1026–1033. doi: 10.1016/j.bbrc.2005.05.191

Figure 2.

Figure 2

Manassantins inhibit hypoxic induction of HIF-1 target genes CDKN1A, GLUT-1, and VEGF in T47D cells. Quantitative real time RT-PCR analysis was performed to determine CDKN1A (A), GLUT-1 (B), and VEGF (C) mRNA levels following treatments with 1, 2, 4, and 6 (30 nM each) under hypoxic conditions (1% O2) for 16 h. "C" represents media only. Data shown are relative expression levels (mean ± SD) of target genes normalized to that of an internal 18S rRNA control, determined from one representative experiment performed in triplicate.