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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biotechnol Bioeng. 2010 May 1;106(1):9–17. doi: 10.1002/bit.22652

Figure 4.

Figure 4

Expression of 6D8 mAb in N. benthamiana plants. N. benthamiana leaves were either co-infiltrated with separate vectors for light chain (pBY-L(6D8)),heavy chain (pBY-H(6D8)), REP110 and P19 (blue bars), or infiltrated with a single vector harboring replicons for light, heavy chain and Rep (pBY-HL(6D8).R, red bars). Leaves were also infiltrated with a construct encoding the mRNA silencing inhibitor P19 as a negative control. Agroinfiltrated leaves were harvest on days 2, 4, 6, and 8 dpi and protein extracts were obtained by homogenizing leaves with extraction buffer (Materials and Methods). Clarified leaf protein extracts were analyzed with an ELISA that detects the assembled form of 6D8 mAb with a goat anti-human gamma heavy chain specific antibody as the capture antibody and a HRP-conjugated anti-human-kappa chain antibody as the detecting antibody. Data are means ± SD of samples from three independent infiltration experiments.