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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biotechnol Bioeng. 2010 May 1;106(1):9–17. doi: 10.1002/bit.22652

Figure 6.

Figure 6

Formation of two separate replicons by a single vector. DNA from leaves infiltrated with various vector combinations as indicated was digested with XhoI, run on 1% agarose gel and stained with ethidium bromide (A), blotted onto membrane, and detected with a GFP probe (B) or a DsRed probe (C). The preparation of GFP- and DsRed-specific probes is described in Materials and Methods. Numbers at left indicate sizes in kbp of DNA molecular weight markers. Note that pBYGFP produced a replicon of ~3kbp (consistent with data in Fig. 3), while the replicon from pBYGFP.R is ~4kbp due to the presence of the C1/C2 gene. The dual replicon construct pBYGFPDsRed.R produces a GFP replicon at ~3kbp and a DsRed replicon containing the C1C2 gene (Fig. 1) at ~4kbp.