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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Biotechnol Bioeng. 2010 May 1;106(1):9–17. doi: 10.1002/bit.22652

Figure 7.

Figure 7

Purification of 6D8 mAb from N. benthamiana leaves. 6D8 mAb from the multi-replicon single vector (pBY-HL(6D8).R) Infiltrated leaves was purified and analyzed on a SDS-PAGE under reducing conditions. Lane 1, Clarified plant extract ; lane 2, Plant proteins removed by 35% ammonium sulfate precipitation; lane 3, 60% ammonium sulfate pellet fraction resuspended for Protein G Chromatography; lane 4, Protein G flow-through fraction; lane 5, Purified 6D8 mAb in the Protein G eluate; lane 6, Human IgG as a reference standard.