Figure 5.
DC-SIGN and dectin-1 cluster around zymosan particles. DC at a concentration of 5 · 105 cells/ml were layered over glass coverslips and incubated with Alexa-Fluor 488 (green) labeled zymosan particles (5 particles per cell) for different times at 37°C. Anti-DC-SIGN mAb and goat anti-mouse IgG Ab labelled with Alexa-Fluor 594 (red) were used for staining DC-SIGN. Note that upon zymosan stimulation, DC-SIGN clusters around zymosan, but not around engulfed particles inside the cell (arrowheads) (a). DC were treated with anti-dectin-1 mAb and goat anti-mouse IgG Ab labelled with Alexa-Fluor 594 (b). Cells incubated in the absence of zymosan particles were stained for DC-SIGN and dectin-1 (c, d). Cells incubated in the presence of zymosan particles were assayed for CD45 staining at the times indicated (e). Reproduced with permission [53].