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. Author manuscript; available in PMC: 2010 Jul 18.
Published in final edited form as: J Mol Biol. 2010 Feb 1;397(2):467–480. doi: 10.1016/j.jmb.2010.01.052

Fig. 7.

Fig. 7

Thermal stability of pHLA-G complexes. The ellipticity at 218 nm was measured as temperature increased from 20 to 90 °C. The data were normalised to determine the midpoint of thermal denaturation (Tm). Samples were diluted to 5 and 10 μM in 10 mM Tris, pH 8.0, containing 150 mM NaCl. The complexes were measured twice at both 5 and 10 μM, and the Tm was averaged over the four experiments. The figure is representative of denaturation at 5 μM. Data for HLA-GRLPKDFRIL, HLA-GKLPAQFYIL, HLA-GKGPPAALTL and HLA-GRIIPRHLQL are shown in red, orange, green and purple, respectively.