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Validation of the polyclonal antibody against NDCBE. (A) Western blotting of N-termini of NBCn1, NDCBE, and NBCn2 (i.e., NCBE). (B) Western blotting of membrane preparations from oocytes expressing full-length transporter NDCBE-EGFP, NBCn1-B-EGFP, NBCn2-B-EGFP, or oocytes injected with H2O. (C) Western blotting of membrane preparations from oocytes expressing NDCBE-EGFP, NBCn2-B-EGFP, NBCe1-A-EGFP, EGFP-NBCe2, or oocytes injected with H2O.
