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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Apr 9;44(3):282–296. doi: 10.1016/j.mcn.2010.03.014

Figure 2. Optimization of growth conditions for differentiation.

Figure 2

A-H. Map2 (red) staining of P19T1A2 cells treated ± Dox at 0.5 μg/ml for 10 days under the following growth conditions: (1) MEMα (7.5% CS, 2.5% FBS), (2) MEMα (1% FBS), (3) MEMα (7.5% CS, 2.5% FBS) for the first three days of differentiation, followed by a switch to Neurobasal media (B27, GlutaMAX), and (4) OPTI-MEM (1% FBS). Nuclei were visualized with DAPI and appear blue. Under the first three growth conditions, cells differentiated into neurons that were immunoreactive to Map2 in the presence of Dox (E-G), although cells grown in reduced serum were immunoreactive to Map2 protein in the absence of Dox (B). Cells grown in OPTI-MEM resulted in Map2-positive cells that were morphologically less differentiated than the other conditions (H). Scale bar = 100 μm. I. Western blotting for expression of Map2 protein under the growth conditions shown in (A-H). The highest amount of Map2 protein was observed in cells cultivated in Neurobasal media (Condition 3).