Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Apr 9;44(3):282–296. doi: 10.1016/j.mcn.2010.03.014

Figure 5. P19T1A2 cells show characteristics of mature neurons.

Figure 5

A. Western blot analysis examining the protein expression of selected Ascl1-induced target genes identified in the microarrays. As predicted from the microarray results, expression of Gap43, Isl1, Synaptophysin (Syp), and Tau proteins increased in response to Dox-induced overexpression of Ascl1. B. P19T1A2 cells have the electrophysiological properties of neurons. Shown is a representative action potential-like waveform recorded from a P19T1A2 cell grown in the presence of Dox for six days. C, C′. P19T1A2 cells are polarized. Immunostaining for expression of the dendritic marker Map2 (red) and the axonal marker Neurofilament-L (NF-L, green). Nuclei were visualized with DAPI staining and appear blue. The boxed area in (C) is enlarged in panel (C′), with dendritic varicosities indicated by white arrowheads.