Figure 7. A novel Gadd45γ-immunoreactive protein unique to P19 cells is induced during neuronal differentiation.
A, B. RT-PCR analysis of early (A) and late (B) stages of Ascl1-induced neuronal differentiation showed that Gadd45γ mRNA expression increased in response to Ascl1, remained elevated through day four, and then declined. Results are shown as the mean ± s.d. normalized to GAPDH levels. C. Western blot analysis showing induction of Gadd45γ protein expression during Dox-induced differentiation of P19T1A2 cells.