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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2010 Apr 9;44(3):282–296. doi: 10.1016/j.mcn.2010.03.014

Figure 8. Direct transcriptional regulation of Gadd45γ by Ascl1.

Figure 8

A. Co-transfection of the Gadd45γ reporter with Ascl1 yielded a relative luciferase activity of 5.4 as compared to 0.46 in the absence of Ascl1 (a 12-fold increase). Results are expressed as mean relative luciferase activity, with error bars denoting standard deviation. *p < 0.01. B. Transcriptional activity of 5′ Gadd45γ deletion reporters in response to Ascl1. Schematics of pEL2 reporter constructs used are shown on the y-axis. Deletion analyses localized the necessary Ascl1 regulatory element to within 281 bp upstream of the transcriptional start site, and mutational analysis showed that augmentation of Gadd45γ transcription was dependent on the presence of two proximal E-boxes. * p < 0.01 as compared to the wild-type 281-EL2 reporter. C. Co-transfection of the Gadd45γ reporter with Neurog2 yielded a relative luciferase activity of 13 as compared to 2.6 in the absence of Neurog2 (a 5.2-fold increase). Results are expressed as mean relative luciferase activity, with error bars denoting standard deviation. *p < 0.01.