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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Pharmacogenet Genomics. 2010 Jan;20(1):26–37. doi: 10.1097/FPC.0b013e3283343296

Fig 4.

Fig 4

Expression of b5 and b5R immunoreactive protein in three human livers with non-synonymous cSNPs in either CYB5A or CYB5R3. Representative immunoblots shown for assays done in duplicate; 30 µg microsomal protein loaded and probed with rabbit polyclonal anti-human b5 or anti-human b5R antibody. (a) wild-type (WT) CYB5A homozygote, and S5A heterozygote in CYB5A showing absence of b5 expression at 17 kDa. Additional bands (22 and 54 kD) represent as yet unidentified proteins that cross-react with anti-human b5 antibody. (b) wild-type CYB5R3 homozygote, and R59H heterozygote in CYB5R3, showing modest but significantly decreased b5R protein expression (37 kDa), when normalized to β-actin (42 kDa). Separate blot with wild-type wild-type CYB5R3 homozygote, and R297H heterozygote in CYB5R3, showing two bands for the R297H subject.