Table 1.
Primers used for cDNA resequencing and SNP confirmation
| Experiment | Orientation | Primer sequence (5′→3′) |
|---|---|---|
| b5 PCR and resequencing |
Forward | GGCCTGGCTCGCGGCGAACCGAG |
| Reverse | CTCCCGTGTCCAAAGCAGGC | |
| b5R PCR | Forward | ACCATGGGGGCCCAGCTCA |
| Reverse | ACTGGGTGAGCGTGAACAG | |
| b5R resequencing | Forward | CTACCTCTCGGCTCGAATTG |
| Reverse | TGTCTCCAATCTGCATGCTC | |
| Novel SNP confirmation resequencing | ||
| b5 S5A | Forward | GTCGGAGGCCCTTCTACTG1 |
| Reverse | TCTTGCTGTGGTTGTGCTTC | |
| b5R R59H | Forward | TGCTCATGAAGCTGTTCCAG |
| b5R R297H | Forward | TTCTGCACGCTTCAAGCT |
1
anneals to genomic DNA at −159