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. 2010 May 5;30(18):6291–6301. doi: 10.1523/JNEUROSCI.0550-10.2010

Figure 3.

Figure 3.

A reduced DG is associated with a drop in neonatal but not adult neurogenesis. A, B, Proliferating cells labeled with immunohistochemistry for BrdU (A, arrows) or PH3 in 8-week-old brains. BrdU-IR cells appear equally dense in the SGZ and hilus in control and double mutant brains (A). The rectangles in A indicate field size used for counting BrdU-IR and PH3-IR cells. B, Density of PH3-IR cells in the SGZ of control and double mutant brains (n = 3, each group) is not significantly different. C, Coronal sections through the DG hilus at P5 processed with double immunofluorescence for BrdU and the neuronal marker NeuN. Double immunofluorescence (merge) labels twice as many newborn neurons in the DG hilus of a control mouse than a double mutant (the white arrows indicate double-positive cells). D, Density of PH3-IR cells in the hilus of neonatal control and double mutant brains (n = 3, each group). The density of mitotic cells was significantly less in double mutants (p = 0.003, one-tailed t test). Data are represented as means ± SEM. **p < 0.01. Scale bar: (in C) A, 100 μm; C, 50 μm.