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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Hepatology. 2010 Mar;51(3):986–995. doi: 10.1002/hep.23411

Fig. 2. Expression of MAT2A and MAT2β genes is induced during in vivo HSC activation.

Fig. 2

Rats were subjected to BDL surgery or sham operation for a period of 10 days. HSCs were isolated from BDL or sham control rat livers and 1x106 cells were plated on 6 cm plastic dishes for 16 hours. (A) RNA was isolated from BDL or sham HSCs and the expression of MAT2A, MAT2β and Col1A2 mRNA was assessed by real-time RT-PCR. Results represent Mean±SE from four BDL or four sham HSC preparations; *p<0.005 vs. sham control. (B) Total cellular protein was extracted from BDL or sham HSCs as described in Methods and subjected to Western blotting for detection of MAT2A, MAT2β and type I collagen. Representative images and densitometric analysis (Mean±SE) from four BDL or sham preparations is shown; *p<0.05, †p<0.005 vs. sham control.