Figure 2.

Ionomycin-sensitive Ca²⁺ pool in 3xTg and PS DKO hippocampal neurons. A, B, Fura-2 340 nm/380 nm ratio traces are shown for WT and 3xTg hippocampal neurons (A) and for PScDKO hippocampal neurons infected with Lenti-GFP or Lenti-Cre viruses or lentiviral combinations (B). The neurons were moved to Ca²⁺-free media for 30 s and challenged with 5 μm IO. Individual cell traces (gray) and average traces (red) are shown for each group of neurons. C, The average size of ionomycin-sensitive Ca²⁺ pool after 30 s incubation in Ca²⁺ free media (IO₃₀). The area under IO-induced fura-2 signal was integrated for each neuron. In each experiment, IO₃₀ values were normalized to IO₃₀ values measured for the WT group (for 3xTg experiments) or to the GFP-infected group (for PScDKO experiments). The normalized responses from different experiments were averaged and presented as mean ± SE (n indicates number of cells analyzed). The IO₃₀ values in 3xTg neurons were compared with IO₃₀ values in WT neurons, and the IO₃₀ values in PScDKO neurons were compared with IO₃₀ values in the GFP-infected group. **p < 0.01; ***p < 0.001. n.s., Not significant.