Figure 7.

RyanRs compensate for impaired ER Ca²⁺ leak function in 3xTg hippocampal neurons. A, C, Fura-2 340 nm/380 nm ratio traces are shown for WT and 3xTg hippocampal neurons in control conditions (Ctrl) or after preincubation with 50 nm dantrolene (Dan) (A) and for WT and 3xTg hippocampal neurons infected with control shRNAi lentivirus (Ctrl) or RyanR-targeted shRNAi lentivirus (RyR) (C). The neurons were moved to Ca²⁺-free media for 30 s and challenged with 5 μm IO. Individual cell traces (gray) and average cell traces (red) are shown for each group of neurons. B, D, The average size of the ionomycin-sensitive Ca²⁺ pool after 30 s incubation in Ca²⁺-free media (IO₃₀). The area under IO-induced fura-2 signal was integrated for each neuron. In each experiment, IO₃₀ values were normalized to IO₃₀ values measured for the WT Ctrl group. The normalized responses from different experiments were averaged and presented as mean ± SE (n indicates number of cells analyzed). ***p < 0.001. ns, Not significant.