Figure 5. Dicer loss induces apoptosis of established B cell lymphomas.

(A-E) Lymphomas from Dicer^fl/fl/Eμ-myc transgenic mice infected with CreER^T2 encoding retrovirus or an empty retrovirus (Vec). 4-OHT or vehicle control (ETOH) was added to lymphoma cell cultures at day or time 0 and cell number (A), viability (B), apoptosis (percentage of cells in subG1 indicated, C), and Dicer gene rearrangement (D) were evaluated at intervals. A, B, and C contain representative data from a minimum of three independent experiments. For D, data for four independent Dicer^fl/fl/Eμ-myc CreER^T2 expressing lymphomas are shown. (E) Dicer rearrangement PCR of FACS sorted single lymphoma clones that emerged following 4-OHT activation of CreER^T2. Nineteen of 43 total clones analyzed are shown. For D and E, a Dicer^fl/fl (D^fl/fl) MEF with or without Cre recombinase serves as a control for Dicer deletion.