Figure 2.

Comparison of cells expressing the C-terminal domains of moesin, ezrin and radixin. NIH3T3 cells were transfected with C-terminal domain-GFP fusion proteins of ezrin and radixin As with moesin, no effect on cell behavior is seen 6 hours after transfection (a,b) Similar to C-moesin and consistent with identical amino acid sequences of the F-actin-binding region, both C-ezrin and C-radixin bind to actin filaments in stress fibers (sf) (b,c,e,f) and numerous microextensions. This localization is sensitive to cytochalasin D.