Table 2.
Primer # | Template | Cassette created | Direction | Oligonucleotide sequence (5′ → 3′) |
---|---|---|---|---|
K4125 | N16961 genomic DNA | PargC | forward | caggatcctaagtggtgaaagagagcgaaca |
K4126 | N16961 genomic DNA | PargC | reverse | tgggaattcgcggccgctatctttgctcctgctattgacagagtg |
K4245 | N16961 genomic DNA | Pvca1008 | forward | gaattcgcggccgctaatttcgattcctcatcactcaccacggcg |
K4246 | N16961 genomic DNA | Pvca1008 | reverse | ggatcctattagtttgattgggctttac |
K5512 | N16961 genomic DNA | PfhuC | forward | gaattcgcggccgcggtgccttaatgaaagtaattg |
K5513 | N16961 genomic DNA | PfhuC | reverse | ggatccattgcgcatgataaaacctctgc |
K5917 | pSE380 (Invitrogen) | rrnB-Pneg | forward | caattgataaaacagaatttgcctgg |
K5918 | pSE380 (Invitrogen) | rrnB-Pneg | reverse | ggatccattcttatgaattcgagtttgtagaaacgcaaaaag |