Figure 3.

Expression of Gγ8-tgOR continues even after downregulation of Gγ8-TTA. Double-label in situ hybridization was used to analyze expression of TetO-P2 (A, red) or TetO-GFP (B, red) and Gγ8-TTA (A, B, green) in the MOE of 3-week-old mice. The expression of Gγ8-P2 (A, red) illustrates that tgORs are expressed in OSNs containing TTA but, importantly, persists in many more mature (apical) neurons where Gγ8-TTA is no longer detectable. In contrast, Gγ8-GFP expression (B, red) closely matches that of Gγ8-TTA; the basal and apical extent of the epithelium are indicated by dotted lines in the left panels of A and B. Scale bar, 20 μm.