Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Mutant human SPT heterotrimers synthesize 1-deoxySa (doxSA). Long-chain bases were analyzed from yeast lcb1Δlcb2Δ mutants expressing wild-type (broken line) or mutant (solid line) hLCB1/hLCB2a/ssSPTa (A) or hLCB1/hLCB2a/ssSPTb (B) heterotrimers or from CHO-LyB cells expressing wild-type (broken line) or mutant (solid line) hLCB1/hLCB2a/ssSPTa (C) or hLCB1/hLCB2a/ssSPTb (D) heterotrimers. The C18-1-deoxySa (C18 DoxSa) eluted at ∼36 min and the C20 DoxSa at ∼73 min.
