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Effect of assay conditions on RNase BN specificity. [3′-32P]pC-labeled pre-tRNASelC (0.04 μm) was treated with RNase BN (0.14 μg) in the presence of Co2+ at pH 6.5 or pH 7.5 (A) or in the presence of Mg2+ at pH 6.5 or pH 7.5 (B). Cleavage products were analyzed by 20% denaturing PAGE.
