FIGURE 2.

Fatty acid composition at the sn-2 position of leaf glycerolipids (lyso-derivatives). Individual lipids were purified by two-dimensional TLC on Silica Gel 60, eluted from the gel, and incubated with Rhizopus lipase as described under “Experimental Procedures.” The resulting lyso lipids (representing the sn-2 position of the parent lipid) were purified by TLC and derivatized using 3 n methanolic HCl, and the resulting fatty acid methyl esters were analyzed by gas chromatography. C16 represents the sum of 16:l, 16:2, and 16:3; C18 represents the sum of 18:0, 18:1, 18:2, and 18:3. A, MGDG; B, DGDG; C, PG; D, PC. Error bars represent mean ± S.D. (n = 3). Statistical significance compared with the wild-type (WT) was determined by the two-tailed Student's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001).