TABLE 2.
Glycerolipid profile and fatty acid composition of leaf lipids from wild-type (WT) and gpsAFR transgenic lines
Lipids were extracted from leaf material of 3-week-old plants. The polar lipids were separated by thin layer chromatography, and fatty acid compositions were determined by gas chromatography of methyl esters obtained by derivatization of individual lipids using 3 n methanolic HCl. Lipids were quantified by adding 10 μ g of 17:0 as an internal standard prior to derivatization. Values are expressed as mean ± S.D. (n = 3 to 5).
| Glycerolipid | Total polar lipids | Fatty acid composition |
C16:C18 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 16:0 | 16:1a | 16:2 | 16:3 | 18:0 | 18:1 | 18:2 | 18:3 | |||
| % | mol % | |||||||||
| MGDG | ||||||||||
| WT | 39.1 ± 3.6 | 0.9 ± 0.3 | 1.3 ± 0.1 | 1.7 ± 0.1 | 38.8 ± 2.0 | 0.2 ± 0.1 | 1.0 ± 0.1 | 2.9 ± 0.5 | 53.3 ± 3.2 | 0.74 ± 0.07 |
| 22–44 | 45.6 ± 3.1b | 2.2 ± 0.4b | 1.0 ± 0.3 | 1.7 ± 0.2 | 43.0 ± 3.4b | 0.3 ± 0.1 | 0.6 ± 0.3 | 1.8 ± 0.4 | 49.4 ± 1.9 | 0.92 ± 0.09b |
| 102–5 | 49.3 ± 2.1c | 2.2 ± 0.5b | 1.3 ± 0.2 | 1.7 ± 0.6 | 41.6 ± 2.1b | 0.5 ± 0.2 | 0.7 ± 0.6 | 3.5 ± 2.1 | 48.6 ± 3.1 | 0.88 ± 0.03b |
| DGDG | ||||||||||
| WT | 17.1 ± 1.3 | 14.6 ± 0.2 | 0.3 ± 0.2 | 0.7 ± 0.1 | 3.0 ± 0.3 | 1.2 ± 0.5 | 0.9 ± 0.3 | 4.9 ± 0.7 | 74.4 ± 1.1 | 0.23 ± 0.01 |
| 22–44 | 13.3 ± 0.9b | 25.0 ± 1.5d | 0.4 ± 0.1 | 1.0 ± 0.1 | 5.0 ± 0.8b | 2.6 ± 0.1b | 0.9 ± 0.1 | 4.8 ± 0.5 | 60.2 ± 0.9d | 0.46 ± 0.01d |
| 102–5 | 14.9 ± 1.7b | 24.0 ± 3.0c | 0.5 ± 0.1 | 0.9 ± 0.2 | 4.4 ± 0.5b | 2.5 ± 1.2 | 1.2 ± 0.4 | 5.4 ± 0.9 | 61.1 ± 4.1c | 0.43 ± 0.06c |
| Sulfoquinovosyl diacylglycerol | ||||||||||
| WT | 2.7 ± 0.7 | 46.7 ± 1.2 | 2.5 ± 0.6 | 2.0 ± 0.4 | 7.2 ± 0.9 | 41.6 ± 0.7 | 0.88 ± 0.04 | |||
| 22–44 | 2.5 ± 0.2 | 62.3 ± 0.5d | 4.2 ± 0.3b | 0.7 ± 0.7b | 4.2 ± 0.3c | 28.6 ± 1.3d | 1.66 ± 0.04d | |||
| 102–5 | 2.5 ± 0.4 | 54.6 ± 4.1b | 6.2 ± 3.4 | 1.7 ± 0.8 | 5.2 ± 1.3 | 32.4 ± 5.3b | 1.20 ± 0.21b | |||
| PG | ||||||||||
| WT | 11.7 ± 1.4 | 30.2 ± 5.9 | 26.8 ± 2.3 | 1.1 ± 0.3 | 4.9 ± 0.4 | 9.9 ± 1.4 | 27.1 ± 3.3 | 1.33 ± 0.20 | ||
| 22–44 | 12.8 ± 1.8 | 42.0 ± 6.5 | 26.5 ± 4.9 | 2.8 ± 0.3c | 3.2 ± 0.6b | 5.4 ± 1.2c | 20.1 ± 2.1b | 2.18 ± 0.15c | ||
| 102–5 | 13.8 ± 0.8 | 35.4 ± 3.8 | 28.4 ± 1.3 | 2.6 ± 0.6b | 4.0 ± 1.2 | 6.8 ± 2.0 | 22.8 ± 2.9 | 1.78 ± 0.23b | ||
| PC | ||||||||||
| WT | 16.6 ± 0.4 | 25.9 ± 2.4 | 2.3 ± 0.6 | 4.6 ± 0.7 | 30.2 ± 4.1 | 36.9 ± 1.0 | 0.35 ± 0.04 | |||
| 22–44 | 13.4 ± 1.4c | 35.4 ± 4.8b | 5.9 ± 1.5b | 2.1 ± 0.1c | 21.2 ± 3.1b | 35.4 ± 3.5 | 0.55 ± 0.11b | |||
| 102–5 | 9.5 ± 0.9d | 33.3 ± 4.5b | 5.5 ± 2.4b | 2.6 ± 0.3b | 23.1 ± 4.1c | 35.6 ± 3.5 | 0.5 ± 0.10b | |||
| PE | ||||||||||
| WT | 10.4 ± 1.9 | 32.9 ± 2.9 | 2.1 ± 0.5 | 3.3 ± 1.4 | 34.6 ± 3.6 | 27.2 ± 1.5 | 0.49 ± 0.06 | |||
| 22–44 | 9.6 ± 1.5 | 38.4 ± 4.1 | 3.5 ± 0.7b | 1.1 ± 0.6b | 26.1 ± 3.3b | 30.9 ± 1.8b | 0.62 ± 0.10 | |||
| 102–5 | 8.4 ± 0.9b | 36.7 ± 5.1 | 5.2 ± 1.1 | 1.4 ± 0.6 | 27.6 ± 4.8b | 29.0 ± 4.6 | 0.58 ± 0.12 | |||
| PI | ||||||||||
| WT | 3.4 ± 0.7 | 50.1 ± 3.5 | 2.8 ± 1.3 | 1.6 ± 0.2 | 20.8 ± 3.6 | 24.7 ± 3.1 | 1.00 ± 0.15 | |||
| 22–44 | 3.5 ± 0.5 | 54.0 ± 0.9 | 3.7 ± 1.1 | 0.9 ± 0.1b | 17.2 ± 2.1 | 24.2 ± 0.1 | 1.18 ± 0.04 | |||
| 102–5 | 3.2 ± 1.2 | 49.8 ± 5.2 | 5.4 ± 1.5b | 1.9 ± 0.9 | 18.5 ± 6.3 | 24.5 ± 4.9 | 0.99 ± 0.21 | |||
a Represents sum of 16:1 cis and trans in MGDG and DGDG, in PG represent 16:1 trans.
b Statistical significance compared with WT was determined by the two-tailed Student's t test, p < 0.05.
c Statistical significance compared with WT was determined by the two-tailed Student's t test, p < 0.01.
d Statistical significance compared with WT was determined by the two-tailed Student's t test, p < 0.001.