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Strand joining activity of Flp(R308A,Y343G) in the MeP half-site substrate by potential direct attack of the DNA backbone by the 5′-OH. A, joining reactions with Flp(R191A,Y343G) and Flp(R308A,Y343G) were performed and analyzed as described for Fig. 4. The plots below represent three independent experiments. B, schematic diagram for a potential strand joining reaction.
