TABLE 1.

Oligonucleotides used for assembling the substrates used in this study

The scissile phosphate or methylphosphonate position is indicated by (p) or (mp), respectively. A full-site substrate was obtained by PCR amplification of a plasmid pY9, containing a copy of the Flp recombination target site (FRT) using primers 1 and 2.

Oligomer	Sequence
MeP half-site (top strand)	ACT TGG ATC CGA AGT TCC TAT AC(mp) TTT
P half-site (top strand)	ACT TGG ATC CGA AGT TCC TAT AC(p) TTT
P or MeP half-site (bottom strand)	TCT AGA AAG TAT AGG AAC TTC GGA TCC AAG T
Primer 1 (for full-site assembly by PCR)	GGT ATT TCA CAC CGC ATA TGG TGC ACT CTC
Primer 2 (for full-site assembly by PCR)	CTT CTG AGT TCG GCA TGG GGT CAG GTG GG