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DNA agarose gel (2.5%) showing process of high fidelity DNA shuffling of wild-type AtNHX1 gene. Lane M, DL2000, from top to the bottom: 2000, 1000, 750, 500, 250, and 100 bp. Lane 1, 1.6-kb fragment of AtNHX1 acquired by PCR; lane 2, DNA fragments after 2 min of DNaseI digestion in the presence of Mn2+, white box indicates the 100–200-bp fragments that are recycled; lane 3, fragment reassembly with Pfu polymerase; and lane 4, PCR amplification of the reassembly with Pfu polymerase.
