FIGURE 3.

Expression of WSP-1 in neurons, but not muscle or hypodermal cells, rescues aldicarb sensitivity phenotype of wsp-1(gm324) mutant worms. Transgenic worms expressing the wsp-1 gene in neurons (tqEx39), body wall muscles (tqEx37), and hypodermal cells (tqEx40) in the wsp-1(gm324) background assayed for their sensitivity to paraquat. Synchronized 1-day-old adult worms were placed on plates containing 1 mm aldicarb and assayed for paralysis over time (minutes). The neuronal transgene expressing WSP-1 (tqEx39) significantly (p = 0.0037, n = 81) rescued the wsp-1(gm324) nematodes (n = 80) because 50% of these nematode were paralyzed at 57.7 min compared with 44.6 min for wsp-1(gm324). The muscle and hypodermal transgenes did not rescue the aldicarb sensitivity of the wsp-1 mutant worms because 50% of the wsp-1(gm324); tqEx37 nematodes (n = 63) were paralyzed at 43.6 min, and the wsp-1(gm324); tqEx40 nematodes (n = 60) were paralyzed at 41.8 min.