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. 2010 May 20;285(30):23105–23114. doi: 10.1074/jbc.M110.124990

FIGURE 6.

FIGURE 6.

p21 remains elevated in 4N populations after Nutlin removal in A549 and MCF7 cells. HCT116, U2OS, A549, and MCF7 cells were treated with 10 μm Nutlin (Nut) for 24 h followed by Nutlin removal. p21 expression was monitored using flow cytometry at each indicated time point. The analysis was done as described in Fig. 5.