FIGURE 2.

Isothermal titration calorimetry studies for the binding of different dicarboxylic acids to McpS-LBD. A, titration of dialysis buffer (I) and 36 μm McpS-LBD (II) with 6.4-μl aliquots of 1 mm succinate. B, titration of 36 μm McpS-LBD with 1 mm maleate and fumarate. C, titration of 100 μm McpS-LBD with 1 mm malate. Upper panels contain the titration raw data and the lower panels show the integrated and dilution-corrected peak areas of the raw data. Data were fitted with the “one binding site model” of the MicroCal version of ORIGIN (Amherst, MA).