FIGURE 5.

Qualitative capillary assays of P. putida toward the 7 McpS-LBD ligands identified in vitro. The mcpS mutant strain carrying pRK415 (empty plasmid) and the complemented mutant strain carrying mcpS on plasmid pRK415 were used for analyses. Photographs were taken after 5 min except those for the isocitrate (after 20 min) and butyrate (after 10 min). In some cases the agarose in the capillary receeded 1–2 mm during the assay and cells were accumulated in the tip of the capillary. Note that citrate, butyrate, and isocitrate were tested at 50 mm to detect a response. CAA, casamino acids.