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Competition between strong and weak attractants in vitro. To elucidate whether both classes of compounds compete for binding to McpS-LBD, ITC competition experiments were carried out. These experiments involved the titration of McpS-weak attractant complexes with strong attractants and, vice versa. A, microcalorimetric titration of 35 μm McpS-LBD with aliquots of 1 mm citrate. B, microcalorimetric titration of 35 μm McpS-LBD, containing 1 mm malate, with aliquots of 1 mm citrate.
