FIGURE 4.
Localization of transiently expressed truncated SYT1-EmGFP proteins in protoplasts. A, schematics of truncated SYT1 with EmGFP (green box) in the C terminus. The TM, SMP, C2A, and C2B domains are represented as yellow, orange, red, and blue boxes, respectively. B, projection images generated from optical sections of EmGFP florescence in protoplasts expressed each truncated SYT1-EmGFP protein. Protoplasts transfected with the plasmid constructs containing the truncated SYT1-EmGFP described in panel A were observed using confocal fluorescence microscopy. The obtained images of each optical section of the protoplasts were reconstructed as projection images. BF, bright field; GFP, GFP fluorescence; CP, fluorescence of chlorophyll; Merged, merged image of GFP with CP. Bars indicate 10 μm.