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. 2010 May 24;285(30):23165–23176. doi: 10.1074/jbc.M109.084046

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Localization of transiently expressed truncated SYT1-EmGFP proteins in ER- or Golgi-labeled protoplasts. A, SYT1-EmGFP and truncated SYT1-EmGFP proteins were transfected into protoplasts isolated from leaves of a transgenic plant expressing CFP-tagged Golgi marker proteins. B, co-localization analysis of SYT1-EmGFP or TM-SMP with ER-Tracker. C, CFP fluorescence in a transgenic plant expressing CFP-tagged Golgi marker and wild-type protoplasts stained with ER-Tracker. Projection images generated from optical sections of EmGFP florescence and CFP or ER-Tracker in protoplasts expressing full-length or truncated SYT1-EmGFP proteins. Emissions of EmGFP and CFP or ER-Tracker were observed using a confocal fluorescence microscope. The fluorescence images obtained from individual optical sections were reconstructed as projection images. SYT1, full-length SYT1; C₂A-C₂B, tandem C₂A and C₂B domains; TM-SMP, TM with SMP, BF, bright field; GFP, GFP fluorescence; Golgi, CFP fluorescence; ER, fluorescence of ER-Tracker; Merged, merged image of GFP with CFP or ER-Tracker. GFP fluorescence was pseudocolored green, and CFP or ER-Tracker fluorescence was pseudocolored magenta. Bars indicate 10 μm.