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. 2010 May 14;285(30):23208–23223. doi: 10.1074/jbc.M109.047464

FIGURE 3.

FIGURE 3.

Panel A, shown is analysis of the effect of TLR3 variants on type I interferon signaling after enterovirus infection (m.o.i. = 0.1). Stable cell lines expressing WT, Ser-554 (P554S) or Phe-412 (L412F) TLR3-HA were transfected with pISRE-LUC and then stimulated infected with Coxsackievirus B3. Luciferase activity was measured at 0 (red bars), 24 (black bars), and 48 h (gray bars) post-infection, normalized to exogenous TLR3 expression, and expressed relative to uninfected cells. The data shown are the means ± S.E. of eight independent measurements. *, p < 0.001 versus all other groups; §, p < 0.001 versus all other groups including WT cells infected for 24 h; ¶, p < 0.001 versus infected WT and Phe-412 cells infected for 24 h; p = NS versus uninfected Ser-554 and both Ser-554 and Phe-412 cells infected for 48 h; #, p < 0.001 versus uninfected Phe-412 and Phe-412 cells infected for 48 h; ∞, p = NS versus uninfected Ser-554 or Phe-412 cells. Panel B, a plaque assay measures the viral progeny titers in the culture media collected 24 (left) and 48 h (right) post-infection of COS7 stable lines expressing WT, Ser-554 (P554S), or Phe-412 (L412F) TLR3-HA with Coxsackievirus B3 (m.o.i. = 0.1). The data shown are the means ± S.E. of three independent experiments. *, p < 0.015 versus infected WT; ¶, p < 0.05; #, p < 0.015 versus infected WT cells.