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. 2010 May 27;285(30):23233–23240. doi: 10.1074/jbc.M110.117457

FIGURE 5.

FIGURE 5.

AGE-mediated up-regulation of Egr-1 in THP-1 cells: dependence on RAGE and mDia-1. A and B, THP-1 cells were subjected to hypoxia for the indicated times. Supernatants were harvested and applied to ELISA for detection of AGE (A) and HMGB1 (B) epitopes. C, THP-1 cells were preincubated with or without anti-AGE IgG (15 μg/ml), or aminoguanidine (AG) (50 and 200 μm) for 2 h and then subjected to hypoxia for 15 min. RNA was isolated from those THP-1 cells for analysis of Egr-1 expression by real-time PCR. D, THP-1 cells were transfected with siRNA-mDia-1 or scramble siRNA and then exposed to CML-AGE (50 μg/ml) for 15 min. RNA was isolated from those THP-1 cells for analysis of Egr-1 expression by real-time PCR. * (p < 0.0001), ** (p < 0.001), and ⋀ (p < 0.01) indicate statistical significance; #, no statistical significance.