FIGURE 5.

miR-93 targets vegfa. A, schematic diagram of vegfa 3′-UTR reporter construct. B, sequence alignment between miR-93 and mouse vegfa 3′-UTR wild type (wt) and miR-93 mutant (mut). Red color indicates the sequence of the mutated miR-93 binding site. C, HeLa cells transfected with either luc-vegfa-wt or luc-vegfa-mutant, along with miR-93 mimics (30 nm). A nonrelated miRNA (miR-690) was used as control. Luciferase activities were normalized to β-galactosidase activities. Results were obtained from three independent experiments. Data are shown as mean ± S.E. (error bars). NS, nonsignificant. D, Western blot analysis of VEGF-A protein levels in cells transfected with miR-93 mimics.