FIGURE 6.

miR-93 represses VEGF-A production in podocytes. A, VEGF released into the culture medium was measured by ELISA after 24 h of exposure to high glucose (HG). Similar experiments were carried out in cells cultured in normal glucose (NG) medium. B, effect of miR-93 inhibitor on VEGF after 24 h of exposure to high glucose was measured by ELISA. C, podocytes were transfected with pEGP-miR-93 plasmid (green), and expression of VEGF (red) was assessed by deconvolution microscopy. Original magnification, ×400. D, quantitative analysis was based on fluorescence intensity of VEGF.