FIGURE 5.

Effect of thiol group oxidation and reduction on TRPC6-mediated Ca²⁺ response. A, Ca²⁺ entry response in TRPC6-expressing cells with treatments of NEM (300 μm) and thimerosal (Thim, 100 μm), and their vehicles (DMSO for NEM and H₂O for thimerosal). *, p < 0.05, compared with corresponding vehicle. n indicates the number of cells analyzed. B, percent increases in the intracellular Ca²⁺ levels upon Ca²⁺ readdition in TRPC6-expressing cells with the treatments of H₂O₂ (10 μm), H₂O₂ plus DTT (1 mm), or H₂O₂ plus GSH-MEE (1 mm). DTT was applied ∼2 min prior to H₂O₂ application. *, p < 0.05, compared with H₂O₂ group. n indicates the number of cells analyzed. Each group in A and B represents the cells from 5 to 6 sets of transfection.