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Lon is required when the Sec translocon is compromised. The Δlon::KanR mutant allele was introduced by bacteriophage P1-mediated transduction into the cold-sensitive mutant alleles secAcsR11 and secY205 (A) or into the temperature-sensitive mutant allele secA51 (B). Transductants were tested for their ability to grow on LB agar plates at the indicated temperatures. The representative set of results presented was obtained in the SG20781 strain background.
