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. Author manuscript; available in PMC: 2010 Jul 19.
Published in final edited form as: Br J Ophthalmol. 2008 Sep;92(9):1269–1274. doi: 10.1136/bjo.2007.132431

Figure 4.

Figure 4

Single immunofluorescent staining of glial cell line-derived neurotrophic factor (GDNF) and GDNF family receptor alpha-1 (GFRα-1) (A), double staining of GDNF and GFRα-1 with p63 (B) and ATP-binding cassette, subfamily G (WHITE), member 2 (ABCG2) (C) in the primary human limbal epithelial cultures established from limbal explants with Hoechst 33342 nuclear counterstaining (blue). GDNF and GFRα-1 were expressed by a small population of cells (A, red). The GDNF and GFRα-1 immunopositive subpopulations (B and C1, red) expressed higher levels of p63 (B, green) and ABCG2 (C2, green). Scale bars, 25 µm. These images are representative of the data summarised in table 1. (D) GDNF and GFRα-1 (green) were expressed by cells at front edge of growing colonies on 3T3 feeder layer on day 10 with propidium iodide nuclear counterstaining (red).