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. Author manuscript; available in PMC: 2010 Jul 19.
Published in final edited form as: Exp Eye Res. 2005 Apr;80(4):581–590. doi: 10.1016/j.exer.2004.11.011

Table 2.

Properties of selected populations of human limbal epithelial cells

ALL RAC SAC NAC
Adhesion time 20 min 2 hr None in 2 hr
Population (%) 100 10·4±1·5 17·3±7·9 69·5±4·1
Label retaining cells (%) 3·15±0·55 16·02±2·45 4·78±1·61 0·53±0·74
Integrin β1+cells (%) 26·6±3·1 57·1±9·8 30·8±3·2 9·7±2·0
p63+cells (%) 15·6±3·4 47·5±8·6 18·8±7·1 6·3±3·5
Involucrin+cells (%) 41·8±5·6 1·8±0·6 30·4±5·5 68·6±6·1
Integrin β1 mRNA ++ ++++ ++ +
ΔNp63 mRNA + +++ + ±
ABCG2 mRNA ++ +++ + ±
Involucrin mRNA + − + +++
Keratin 12 mRNA + − + +++
CFE (%) 2·01±0·51 3·77±0·42 2·45±0·61 0·91±0·32
Confluent days 18–24 10–14 18–24 No confluence

ALL, the unfractionated whole cell population; RAC, the rapidly adherent cells; SAC, the slowly adherent cells; NAC, the non-adherent cells.